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Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum ArchiMer
Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Quere, Claudie; Bruneau, A; Riso, Ricardo; Auffret, Michel; Soudant, Philippe.
Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in...
Tipo: Text Palavras-chave: Harmful algae; Metals; Oysters; Toxin accumulation; Physiological effects.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00169/28071/26286.pdf
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Relationship between valve activity, microalgae concentration in the water and toxin accumulation in the digestive gland of the Pacific oyster Crassostrea gigas exposed to Alexandrium minutum ArchiMer
Haberkorn, Hansy; Tran, Damien; Massabuau, Jean-charles; Ciret, Pierre; Savar, Veronique; Soudant, Philippe.
The complexity of the relationships between Alexandrium minutum (Am.) concentration in the water ([A.m.](w)), Paralytic Shellfish Poisoning contamination in the digestive gland ([PSP](dg)) and valve behavior was explored in oysters Crassostrea gigas. Two experiments were conducted, during which oysters' valve behaviour were analyzed. Oysters, first acclimated for 10-days with the non harmful microalgae Heterocapsa triquetra (H.t.), were exposed to four microalgae mixtures at constant total concentrations of 10 x 10(3) cells ml(-1) (experiment-1) and 5 x 10(3) cells ml(-1) (experiment-2): 100% Am.; 50% A.m.-50% H.t.; 25% A.m.-75% H.t.; 100% H.t. At the end of experiment-2, [PSP](dg) were measured. At 10 x 103 cells ml(-1), the microalgal ingestion decreased...
Tipo: Text Palavras-chave: Harmful algae; Oysters; Behavioral responses; Algae concentration; Toxin accumulation.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00041/15195/12617.pdf
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